Extraction, purification and stability of C-phycocyanin from Arthrospira platensis

C-phycocyanin is a highly valuable phycobiliprotein from Arthrospira platensis. However, its extraction, purification and conservation currently limit commercial use. We review here the most common techniques less conventional methods. Simple incubation in phosphate buffer at neutral pH can give high yields (> 100 mg/g) even protein purity 0.7) if parameters (buffer concentration, temperature, time, state etc.) are chosen correctly. This method preferable to distilled water or acid solutions. Some mechanical, physical thermal treatments improve extraction yield accelerate release of C-phycocyanin. Ultrasound-assisted has been widely studied probes generally higher than baths 30 min. technique be coupled with others, such as freeze–thaw methods, release. Indeed, cycles an efficient destructuring that used alone pretreatment. Bead mills pulsed electric fields also extract C-phycocyanin, but there room for improvement choice operating parameters. For extracted pigments, salting out aqueous two-phase increase phycocyanin grade. More advanced mostly based on chromatography, provide addition improvement. stability dependent principally between 5 7 temperatures below 40 °C, use various preservatives conditioning increases lifetime.